Plant Transcription Factor Database
Previous version: v3.0
Aethionema arabicum
HSF Family
Species TF ID Description
AA10G00315HSF family protein
AA17G00081HSF family protein
AA18G00096HSF family protein
AA19G00216HSF family protein
AA19G00468HSF family protein
AA30G00224HSF family protein
AA32G00404HSF family protein
AA32G01052HSF family protein
AA3G00017HSF family protein
AA3G00228HSF family protein
AA40G00417HSF family protein
AA53G00217HSF family protein
AA57G00047HSF family protein
AA57G00161HSF family protein
AA5G00104HSF family protein
AA61G00665HSF family protein
AA625G00003HSF family protein
AA65G00102HSF family protein
AA6G00029HSF family protein
AA85G00014HSF family protein
AA87G00341HSF family protein
AA8G00241HSF family protein
HSF Family Introduction

Heat stress transcription factors (Hsfs) are the major regulators of the plant heat stress (hs) response. Sequencing of the Arabidopsis genome revealed the existence of 21 open-reading frames (ORFs) encoding putative Hsfs assigned to classes A-C. Here we present results of a functional genomics approach to the Arabidopsis Hsf family focused on the analysis of their C-terminal domains (CTDs) harboring conserved modules for their function as transcription factors and their intracellular localization. Using reporter assays in tobacco protoplasts and yeast as well as glutathione-S-transferase (GST) pull-down assays, we demonstrate that short peptide motifs enriched with aromatic and large hydrophobic amino acid (aa) residues embedded in an acidic surrounding (AHA motifs) are essential for transcriptional activity of class A Hsfs. In contrast to this, class B and C Hsfs lack AHA motifs and have no activator function on their own. We also provide evidence for the function of a leucine (Leu)-rich region centered around a conserved QMGΦL motif at the very C-terminus as a nuclear export signal (NES) of class A Hsfs. Sequence comparison indicates that the combination of a C-terminal AHA motif with the consensus sequence FWxxF/L,F/I/L as well as the adjacent NES represents a signature domain for plant class A Hsfs, which allowed to identify more than 60 new Hsfs from the expressed sequence tag (EST) database.

Kotak S, Port M, Ganguli A, Bicker F, von Koskull-Doring P.
Characterization of C-terminal domains of Arabidopsis heat stress transcription factors (Hsfs) and identification of a new signature combination of plant class A Hsfs with AHA and NES motifs essential for activator function and intracellular localization.
Plant J, 2004. 39(1): p. 98-112.
PMID: 15200645